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KMID : 0380020050200050350
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 5 p.350 ~ p.354
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Medium Composition of Enterobacter cloacae YJ-1 for Maximizing Hydrogen Production
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Lee Ki-Seok
Kang Chang-Min
Jung Sun-Yong
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Abstract
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In order to maximize hydrogen production by Enterobacter cloacae YJ-1, anaerobic hydrogen producing bacteria, the medium composition was optimized. Glucose was better than other carbon sources in hydrogen production and its production was 975.4 mL/L at (w/v) for 48 h. Organic nitrogen sources were more effective than inorganic nitrogen sources and also yeast extract among organic nitrogens was the most effective in hydrogen production. Among metal ions, was most effective, and its production was 1753.3mL/L at (w/v). Addition of amino acid was very effective with compare to another components of medium, and cystein was most effective among them. Under the optimum medium obtained in batch culture, semi-batch culture in order to produce continuous hydrogen was run. The highest hydrogen production was earned at (w/v) of glucose and the amount was 2215.4 mL/L.
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KEYWORD
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Hydrogen production, Enterobacter cloacae YJ-1, medium composition, amino acid, semi-continuous culture
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